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| Genre/Form: | Electronic books |
|---|---|
| Additional Physical Format: | Print version: Appasani, Krishnarao Epigenomics : From Chromatin Biology to Therapeutics Cambridge : Cambridge University Press,c2012 |
| Material Type: | Document, Internet resource |
| Document Type: | Internet Resource, Computer File |
| All Authors / Contributors: |
Krishnarao Appasani |
| ISBN: | 9781139528146 1139528149 9781107003828 1107003822 9781139525756 1139525751 |
| OCLC Number: | 804664843 |
| Notes: | Description based upon print version of record. 525 Detection of chemical reporters by click-chemistry |
| Description: | 1 online resource (574 p.) |
| Contents: | Cover; Epigenomics From Chromatin Biology to Therapeutics; Title; Copyright; Dedication; Contents; Contributors; Foreword; Preface; Part I Basics of chromatin biology and biochemistry; 1 Introduction to epigenomics; X-chromosome inactivation (dosage compensation); Genetic imprinting; Epigetic modifications and their mechanisms; Scope of this book; Part I: Basics of chromatin biology and biochemistry; Part II: Epigenomic imprinting and stem cells; Part III: Epigenomic assays and sequencing technology; Part IV: Epigenomics in disease biology; Part V: Epigenomics in neurodegenerative diseases Part VI: Epigenetic variation, polymorphism, and epidemiological perspectivesCurrent issues in epigenomics; Identical twins; Future developments in epigenomics; References; 2 Epigenetics and its historical perspectives; 21 Introduction; 22 Inheritance in somatic cells; 23 Epimutations and dual inheritance; 24 Cancer and de novo methylation; 25 Chromatin remodeling; 26 Differences between genetic and epigenetic systems; 27 An epigenetic component in long-term memory?; 28 A wider definition of epigenetics; 29 The chronology of epigenetics; References 3 Functional networks of human epigenetic factors31 Introduction; 32 Methodology; 321 Design of reporter cell systems; 322 SiRNA-based knockdown analysis; 323 High-throughput siRNA screening; 324 Considerations for design and analysis of the siRNA screen; 33 Results and discussion; 331 Proof-of-concept study to identify factors and networks that participate in epigenetic gene silencing; 332 Design of siRNA library; 333 SiRNA high-throughput screening; 334 Identities of screen hits; 335 Evaluating position-specific versus epigenome-wide roles for silencing factors 336 Evidence for crosstalk between DNA methylation and histone modifications337 Interpreting output from epigenetic factor screens; 338 Identifying networks of "targetable" epigenetic factors; 34 Summary; Acknowledgements; References; 4 Nucleosome positioning in promoters: significance and open questions; 41 Introduction; 411 Micrococcal nuclease bias; 412 To sequence or not to sequence?; 42 Factors potentially affecting nucleosome positioning; 421 Pitfalls of sequence analysis; 422 Periodicity or artifact?; 423 DNA bendability and nucleosome positioning 424 Nucleosome positioning, histone modifications, and DNA methylation43 Hypotheses; 431 Transcription-coupled nucleosome positioning; 432 Chromatin structure as a cancer lineage classifier; 433 Retrotransposons and nucleosome positioning signals; 44 Concluding remarks; References; 5 Chemical reporters of protein methylation and acetylation; 51 Introduction; 52 Methodology; 521 Synthesis of chemical reporters; 522 In vitro transfer assays; 523 Labeling of cell lysates or living cells; 524 Determining the specificity of acetylation and methylation reporters |
Abstract:
Experts from academia, the biotechnology and pharmaceutical industries introduce biological, medical and methodological aspects of the emerging field of epigenomics.
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